Daily flow cytometer quality control requirements:

• Calibrate and standardize according to the manufacturer's recommendation (software and bead product). 
  • Cellular Immunology Standard CILF-S11, CINM-S6, CIML-S7
• Stain and analyze a normal blood and/or validated commercial control(s) for Non-malignant Leukocyte Immunophenotyping. 
  • Cellular Immunology Standard CINM-S11

Monthly flow cytometer quality control requirements:

• Check fluorescence/PMT linearity. 
  • Cellular Immunology Standard CILF-S12, CINM-S7, CIML-S8

• Stain and analyze a normal blood or validated commercial control for Malignant Leukocyte analysis. 
  • Cellular Immunology Standard CINM-S9

As the manufacturer recommends:

• Clean system fluidics.
• Perform preventive maintenance.
• Verify optical alignment on fixed optical rail flow cytometers.

If an instrument is moved:

• Verify optical alignment.
• Calibrate and standardize according to the manufacturer's recommendations for software and bead product. 
  • Cellular Immunology Standard CILF-S11, CINM-S6, CIML-S7
• Check fluorescence/PMT linearity. 
  • Cellular Immunology Standard CILF-S12, CINM-S7, CIML-S8
• Stain and analyze a normal blood and/or validated commercial control(s).

• These guidelines should also be followed when evaluating instrument performance following an instrument repair. Please refer to the Cellular Immunology Checklist_[1] on the Clinical Laboratory Evaluation Program method approval_[2] webpage for instrument validation requirements.