Derbyshire and Gray Laboratory Gallery
Video Gallery
ESX-1 primarily associates with the old “non-septal” pole. M. smegmatis cells expressing a fusion of msmeg0046::yfp were imaged every 10 mins in a heated growth chamber. The fluorescent fusion protein (red) localizes to a single cell pole, the old pole (Wirth et al., Mol. Micro. 2012).
Image Gallery
Transconjugant colonies with different morphotypes result from a cross between M. smegmatis strains MKD24 and Jucho
A transconjugant morphotype; colonies of a purified transconjugant isolated from a cross between M. smegmatis strains MKD24 and Jucho
A transconjugant morphotype; colonies of a purified transconjugant isolated from a cross between M. smegmatis strains MKD24 and Jucho

An M. smegmatis biofilm containing donor cells, mc2155

A biofilm containing both donor and recipient cells of M. smegmatis

A fluorescent image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dendra.

A phase contrast image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dendra

Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain contacts a gfp reporter, which is activated (green) when donor and recipient cells are in direct cell contact.

Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain contacts a gfp reporter, which is activated (green) when donor and recipient cells are in direct cell contact.